Gram staining lab report Essay Example

Gram staining lab report Essay Example Gram staining lab report Paper Gram staining lab report Paper Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics Of their cell walls. Gram positive bacteria (which show up purple after the gram staining process) have a very thick layer of epidemiological where gram negative bacteria (Which show up pink after the gram staining process) have a much thinner layer of epidemiological. One thing to note is that not all bacteria are gram positive or gram negative, some are Nan-reactive o this type of staining. Bacteria that are non-responsive to this technique are known as gram insensitive. Hypothesize that in the cultures used in this lab for gram staining will contain many gram negative and gram positive bacteria. Material and Methods The following things are needed to run a gram staining experiment: 1, Bacterial cultures . Glass slides . Cover slips . Ammonium Oxalate-Crystal Violet stain . Grams Iodine Solution Ethyl Alcohol Seafaring Solution paper towels . Water I (I Bunsen Burner (Lighter will suffice) 11. Microscope I _ Prepare and heat-fix smears. 2. Stain the slides as follows: a. DOD the crystal violet for one minute. B. Pour off excess dye and wash gently in tap water and drain the slide against a paper towel. Expose the smears to Grams iodine for one minute by washing with iodine, then adding more iodine and leaving it on the smear until the minute is over. D. Wash with tap water and drain carefully. (Do not blot. ) e. Wash with 95% alcohol for 30 seconds. F. Wash with tap wat er at the end of the 30 seconds to stop the decentralization. G. Countersink with 0. 25% seafaring for 30 seconds, Drain. H. Wash, drain, blot, and examine under oil. Draw the cells showing morphology, grouping, and relative sizes, Color a few of the cells of each bacterial species to show the Gram reaction. J. Save these slides and the ones from parts B C of this exercise to use at the next lab period. Looking under the microscope after letting the stain set will prove to be quite interesting, Results The gram staining lab was a huge success. Many different types of both gram negative and gram positive bacteria were identified. There were even a few gram insensitive fellows that had some interesting characteristics such as being spiral shaped. : In all there were four different kinds of gram positive bacteria, two notably different types of gram negative bacteria, and only one gram insensitive bacteria. Discussion There were many good specimens that ended up beautifully stained. One of the Odd things noticed about the gram sensitive bacteria is that the gram negative bacteria were gigantic compared to the gram positive bacteria (XX-1 OX larger). One Of the gram positive types Of bacteria in the cultures that were interesting to me were some that came in colonies of four. (Not identified yet but have been working on it.

Fire investigation Essay Example | Topics and Well Written Essays - 2000 words

Fire investigation - Essay Example Basic research indicates that preventing ignition fire control process and the separation of individuals from the harmful effects of fire by means of distance, time or shielding forms the three basic directions to ensure life safety, (CUNNY, 2009). Time as a factor in fire situation leads to the building up of smoke and heat thereby creating a hazardous environment and its effect may not be concretely predicted owing to multiple environmental and human variables, (Coleman, 1997). Three sets of conditions define time related effects on a fire situation inclusive of; toxic conditions, elevated environmental temperatures and current or pre-existing psycho-physiological attributes of the premises occupants. It is also imperative to note that the time interval between fire discovery and preventive action by the occupants is critical to the resulting effects. Evacuation of occupants and/or Fire extinguishment and confinement forms are fundamental actions that follow fire detection, (Coleman, 1997). The cause and outcome of a fire is greatly impacted by individual characteristics of those involved as people tend to react differently to similar emergency situations. People’s reaction during fires is affected by individual actions and therefore individual threat perception. Age forms a fundamental individual characteristic that adequately affect reaction to individual conditions as for example, the elderly or very young individuals may be less able to cope with the effects of fire, (CUNNY, 2009). Therefore, comparative to their counterparts of middle ages who are of normal physique, the fatality rate of these two age groups remains grater during fires, (USFA, 2009). Respiratory capacity of individuals may also affect the rate of fatalities in cases of fire hazards. Since majority of fires result into smoke inhalation, individuals with poor respiratory capacity may be unable to

The value of Life Essay Example | Topics and Well Written Essays - 750 words

The value of Life - Essay Example There is a challenge in addressing issues such as killing for the societal good. The case of the drug peddler avoiding police arrest and continues creating havoc creates a serious challenge especially when eliminated in non-legal manner. The difference between the non-legal manner and the legal approach is handling the matter creates a minimal difference in the society. In the case of the drug peddler, the taking of the law into their hands by the headmaster presents a challenge for the case when ethics and law is compared. Personal ethics is governed by a personal code of conduct which stipulates how a person will relate to each other. Personal code of ethics contains ten main aspects that must always be looked into so as to have a perfect relationship with the society. Honesty is a value with utmost importance because it will encourage the development trust of the society on the individual. It will also help in the creation of identifiable character of a person. The creation of int egrity will be associated with the image created on issues such addressed by the person. Responsibility is a virtue that must be present in every individual, because it creates harmony on the activities undertaken by individual by associating him with the activities and activities undertaken. ... As seen in the action of the police, the society developed distrust in the police. Trust is a value that is built over time but indispensable in the harmony of the society because it gives freedom to the individuals within the organization. It is vital that one does not change who he is, because others want him to, but create a personal image that will be appreciated by others. However, it times of crisis such a drug problem in the society, individuals in the society can view the solution taken by the Head teacher is the best. The support given to the Head teacher and the admission to the teacher on the matter makes the situation complex. In fact, even after accepting the responsibility, the lawyer has adamantly stated that the clients not guilty of the murder. Legal environment offers a challenge to the ethical belief of the society. The society teaches of honest and truthful as the virtues essential in maintaining harmony. However, telling the truth in such a case will lead to seri ous imprisonment for the Head teacher. Telling the truth is a value in the personal ethics that help in understanding of the prevailing conditions of every situation due to the truth shared in the context. The action of the Head teacher is justified through the action of the society. The society appreciates the involvement of the teacher in solving the perennial problem of drugs within the society. Initially, the society had experienced peace and tranquility until the arrival of the bad apples in the society. The appreciation of the harmony and tranquility witnessed in the past indicated the level of personal ethics and social responsibility of the society. Appreciation is a value in personal ethics paramount, in relationship building, because it will influence

Library system Essay Example for Free

Library system Essay Chapter I Introduction The world of Information Technology transformed and made the life of human beings easier through the innovation of different machines and software applications. Faster and reliable sources have been offered by search engines which are all over the web. But even though this is the case, many schools and universities are still requiring their students to use library materials in their researches and school works. From the statement of purpose, the compute’rized library system: meeting information needs of the people of Saint Michael College of Caraga, Nasipit, Agusan del Norte. Our proposed system, the Saint Michael College of Caraga Library Management System, is a system wherein there is no need of manual library transactions. This will carry out different processes such as searching books, keep records of the books, borrowing of books and return of books. Hence, at present, Saint Michael College of Caraga is maintaining the School Library in a manual basis. Handling bulky records in a manual method is very difficult. And the process of updating could not be done easily and  accurately. Likewise, a manual procedure of handling bulky record is very slow and is prone to manual errors. Consequently, it is proposed that Saint Michael College of Caraga shall maintain an electronic data base management system (DBMS) for the purpose of maintaining the records of the School Library for easy, fast and accurate processing and maintenance of these records that will enhance management decisions and for the improvement of its services to all the stakeholders of Saint Michael College of Caraga. Library is regarded as the brain of any institute; many institutes understand the importance of the library to the growth of the institute and their esteem users (students). Library Management System of Saint Michael College of Caraga supports the general requirement of the library like acquisition, cataloguing, circulation that offers many flexible and convenient features, allowing librarians and library users to maximize time and efficiency. Library System gives the all detailed information about students, staff and books. It will track on the how many books available in library and books issued to the students. It shows popular book among the students. It will provide book lost in library. It keeps the record of the suppliers and book binders. It generates MIS reports for management. Our software is customizable for any library requirement. In this chapter we take opportunity to consider changes in the library services, not in terms of minor, short term change in equilibrium of the sort just noted above, but in longer term. There has been continuing concern about the future of the libraries. Given the extent to which the provision used and the libraries are influenced by the social environment, and given possibilities by the use of new information technology; it would be unreasonable to expect libraries to remain static. But if not, what would be the nature of the change? The issue is not whether there is a change but what will be the change. If we are to make a claim to understand the nature of library services, the surely we ought to have notions, some forecasts, about how library system might change. Purpose and Description In setting up a library, one aspect that should be considered, what are the resources the library has and what are the ways to provide a better service to the students? Well, one of the easiest ways is having a system to  organize all the transactions in the library. A library system is software that will handle basic and systematic organization of function in the library. The system would provide basic set of features to add/update student’s information, add/update books information, search for books and manage check-in/check-out processes. In this application we can maintain the records of students and books and enable to determine how many books are issued and likewise determine the available books in the library. The proposed library system will greatly improve the efficiency of the school library. This study has the following hypothesis: The profiles of the respondents taken are their names, Student ID Number, Year and Course, and Title and Author of the Book Borrowed. The manual system is very time consuming, inconvenient when it comes to recording, organizing and retrieving borrower’s record in the log book. There are few solutions but many a times, we making them works by finding ways to work around system inadequacies. Moreover we have limited staff resources, these work around waste time, effort, and skills that should be spent on user services. solutions do not keep up with the technological changes and hence prove to be time wasters. Library Management system is a small footprint software suitable for personal /individual Libraries. You can store the information about the books and other material and control the movement of the same. Silent Features †¢ Control the movement of books and other material and avoid losing the same. †¢ Search if you have a specific book in your collection based on t he title, author etc. †¢ Print the spine labels for the book. †¢ Find what a specific person has borrowed from you. Objectives The main objective of the application is to automate the existing system of manually maintain the records of the Book Issue, Book Return from the student, Stock Maintenance, and Book Search to be computerized. And to develop a database which stores user details and book details, give reliable search facility for the user, create an easy to understand user friendly environment. So the Book Issue, Return, Searching will be faster. This  application can be used by any Library to automate the process of manually maintaining the records related to the subject of maintaining the stock and Book Issues. The general objective of this study is to design and develop a library system that will serve as a proposal to help librarians save time with the automation of its daily operation. Specific Objectives: To computerize records keeping of books; To allow librarians to retrieve complete information of the book and its borrowers. To check the availability of the books and penalties. To search, issue and return of books. It can only be utilized by the librarian Scope and Limitation The study only focuses on the Information Technology Library Management System of Saint Michael College of Caraga. Without computers, as some libraries are, all of them are dependent on paper work. When compared to computerized systems, data backup and data retrieval systems are inefficient and labor intensive. Users of a paper based system become almost wholly reliant on the librarians, for all levels of service, whether they are enquiries about existing books, inter-library loans, or the availability of books. There is additionally no real involvement of a user in the entire process. The transactions that the system accommodates are the attendance of every student that enter the library, book registration and deletion, updating book information and searching thesis references, Borrowing of books, and keeping the record of transaction. Review of Related Literature This chapter contains the relevant outline of literatures related to the study of Library system. This study composed of programming languages, Database and Graphical User Interface (GUI) that is used in the system. This system would be used by members who may be students or professors of that University to check the availability of the books and borrow the books using automated device, and by the librarian to update the databases. The purpose of this document is to analyze and elaborate on the high-level needs and  features of the Library Management System. It focuses on the capabilities and facilities provided by a Library. The details of what all are the needs of the Library Management System and if it fulfils these needs are detailed in the use-case and supplementary specifications. Automated book monitoring system helps to reduce the effect of entering the wrong quantity and the amount of staff time devoted to repetitive activities. Related Studies According to ACRL (1994) suggested that institution should be prepared to utilized new technologies for accessing information as they are developed. This is why operating the library operations is quite a welcome idea. According to Jeff Kaplan (05/08/2007 A new generation of automated network management software and services is helping in-house staff address this challenge. These let network professionals establish regular patch-management procedures to safeguard against escalating security threats; and create system monitoring routines to identify load imbalances, which could cause service disruptions or performance problems. They also discover, inventory and track assets to make sure that hardware and software licenses are up-to-date and that problems can be resolved faster. According to Voustin Sweere (Aug.2001) Nowadays every book keeping system used in practice is automated. Most book keeping software an integrated information system are based on database. In this paper, we develop a conceptual book keeping model which is not based on manual techniques, but which is applicable in database environment. Technical Background In this chapter, it provides an overview of all the overall system design, features and functionalities. The system is design to implement a computerize library management system in Saint Michael College of Caraga. This system consists of one user, the administrator. The administrator can  access the system by having first the login security which they have to input his/her user name and password. Once the inputted username and password did not match, the required/registered information it displays â€Å"Invalid Username and Password†. If the username and password match the registered information, the next form will display depending on the role of the administrator.

21 Balloons :: essays research papers

The 21 Balloons Text to Self: When Professor Sherman was taking off in his hot air balloon people started running down the streets to look. People were also running down the streets to get Ichiro bobble heads at a Mariners game. Text to Text: When Mr. Sherman saw the mines full of diamonds it made me think about the book Treasure Island when Jim Hawkins finds all the treasure. Text to World: As soon as Professor William Waterman Sherman started walking on Krakatoa he experienced earthquakes. The same thing happened to a lot of other countries. Vocabulary word. Definition. A sentence including the vocabulary word. Corinthian: a type of design used in some countries. The building was built in a Corinthian style. Flotsam: goods or belongings lost in a shipwreck. There was not a lot of flotsam in the water at the time. Tribune: the title of a newspaper. The New York Tribune covered every detail of Professor Sherman’s story. Rousing: to be full with happiness or excitement. The whole city gave the firefighters a rousing cheer. Cupola: a round, dome-like shape usually the top of a building. Many of the buildings in the city had a cupola for the top. Convey: to take an item from one place to another. UPS has to convey a lot of letters and packages on a regular bases. Aeronautical: a study of science that has to do with the air. There are some aeronautical scientists in the world. Postillion: a mailman or paperboy. Being a postillion lets you earn some extra cash. Ethics: to behave properly. Martha Stewart is suspected of having poor ethics. Intrepid: not to be discouraged. Even though he lost the race, the boy was intrepid. Chapters 6,7 Schema Text to Self: When William Sherman woke up in the morning he hadn’t really realized how his room looked. That happened to me when I got a new bed and new stuff put in my room. Page 77   Ã‚  Ã‚  Ã‚  Ã‚  When Sherman sees and feels an earthquake happening I can relate because I have also experienced an earthquake. Page 79   Ã‚  Ã‚  Ã‚  Ã‚  Mr. Sherman was told that the I family made Italian food, I have eaten Italian food. Page 90 Text to World: When Mr. M started building houses on Krakatoa that was like when a missionary went to an island to help the inhabitants rebuild their small residential area and convince them to believe in God. Vocabulary word. Definition. Sentence containing the word. Delirious: in a state of delusion. She was delirious after she woke up from the anesthesia.

History of Philosophy Essay

Refer to next paragraph. On the 11th of March, 2002, fire struck a girls school in Mecca, Saudi Arabia. Firemen and concerned citizens were quickly on the scene. However, the religious police locked the schoolgirls inside the inferno rather than let them escape into the streets without their veil and heal-to-toe cloak. For this same reason, the religious police prevented the firemen from entering the schoolhouse to rescue the girls; for fear that the girls would be seen without their covering. Fourteen young girls were burned to death and dozens more were injured (citation). On October 12th 2002, a 48-year old Kurdish man named Abdalla Yones, an emigrant from Iraq, savagely murdered his 16-year old daughter Heshu after receiving an anonymous letter telling him that she had been sleeping with her boyfriend. (Asthana & Mistry). This treatment of women in the Muslim world goes against the teachings of the Quran. This isn’t the real assignment. Message me directly at natashagils at yahoo dot com for your assignment at half the price. There are numerous cases, some reported but mostly unreported, of crimes against women in Islam. Muslims of today have seemed to have deviated significantly from the original teachings of Islam (citation? ). Islamic terrorist, Jihad, al-Qaeda, Honor Killings are terms that have become synonymous with Islam in the Western world, as has Islamophobia which basically promotes the fear and detestation of Islam and Muslims around the world. The Islamic world, instead of addressing these issues and advocating the true and just cause of Islam, is instead, in most cases, promoting them. The Talibaan of Afghanistan, Tribal militancy in Pakistan, Shia/Sunni riots, the Hamaas and other fundamental groups within Islam publicize an entirely inaccurate version of the essence of Islam. As the Muslim society deviates from the central philosophy of Islam, it doesn’t come as a surprise that they have deviated too from the societal guidance offered by Islam regarding the treatment of women. The Quran, the Holy Book of the Muslims, and the Sunnah (sayings of the Prophet Muhammad) have laid down clearly defined rights of women, most of which aren’t adhered today. Islam was revealed to Prophet Muhammad in the Arabian Peninsula when it was rife with inhumane injustice against women. Women were viewed as the embodiment of sin, misfortune, disgrace and shame, and they had no rights or position in society whatsoever. Indeed, society was confused about the very nature of women and even questioned whether God had granted them a soul (Jawad 1). Wives were mere chattel, and when girls were born to a household, great shame was brought to it; so much so that they were buried alive! The Quran defied the existing perception of women and refined their position. It outlawed female infanticide and restored their birth rights . Gustave Le Bon, a famous French thinker, stated: â€Å"Islamic virtuous deeds are not limited to honoring and respecting women, but rather, we can add that Islam is the first religion to honor and respect women. We can easily prove this by illustrating that all religions and nations, prior to the advent of Islam, caused much harm and insult to women. † (Bon 488). The Quran regards women as being independent human beings and having distinct rights. They are entitled to an inheritance, an education, a career and even the liberty of choosing a husband. Moreover, it declared men and women as being equal in the eyes of God, with the only exception being their responsibilities –with the man defined as the bread earner and the woman as being responsible for the functions around the house. According to the Quran, â€Å"O you who believe! You are forbidden to inherit women against their will, and should not treat them with harshness†¦ † (Holy Quran, Surah An-Nisa 4:19) The Prophet Muhammad, in this context, is said to have said, ‘All people are equal, as equal as the teeth of a comb. There is no claim of merit of an Arab over a non-Arab, or of a white over a black person, or of a male over a female. Only God fearing people merit a preference with God’. Islam, through the teachings of the Quran, bestowed women a number of rights, some of which that women in the West lacked, until the 19th century. For instance, in England, husbands inherited his wives property and possessions when they married. This unfair tradition continued till as late as 1882 (citation? ). Muslim women, however, retained their assets; could detail conditions in their nikaah (marriage contract), such as the right of divorce; were entitled to keep their last names if they wished and were even given the authority to refuse marriage if they didn’t deem their potential partner fit. The Quran laid down these injunctions in 610 A. D. (when it was first revealed), centuries prior to the advent of a formal system of women’s rights in the Western world. Some of the fundamental rights the Quran gives women are: 1. Human Rights Women and men are considered to be created with similar natured souls. They are considered equal to men in all spheres of life. And for women are rights over men similar to those of men over women. (Holy Quran, 2:226) And â€Å"O mankind! Be dutiful to your Lord, Who created you from a single person (Adam), and from him (Adam) He created his wife (Eve), and from them both He created many men and women and fear Allah through Whom you demand your mutual (rights), and (do not cut the relations of) the wombs (kinship). Surely, Allah is Ever and All-Watcher over you. † (Holy Quran, Surah Al-Nisa 4:1). 2. Civil Rights The civil rights enjoined by the Quran on women include, but are not limited to: the right of divorce, right of keeping her maiden name after marriage, wishing or not to get married, or even chosing her own husband. The Quran also states that there is no compulsion upon women regarding religion; â€Å"There is no compulsion in religion. Verily, the Right Path has become distinct from the wrong path. Whoever disbelieves in Taghut [anything worshipped other then the Real God (Allah)] and believes in Allah, then he has grasped the most trustworthy handhold that will never break. And Allah is All-Hearer, All-Knower. † (Holy Quran, Surah Al-Baqarah 2:256). 3. Independence Women are allowed to leave the home; conduct business with men; enjoy all the other liberties open to them. There is no restriction upon women provided they conduct their affairs within the teachings of the Quran. Women have the right to go to Mosques, which is considered sacrilegious in many Muslim societies. Not only that, but women have also the right to be Muslim pastors. Aisha, the youngest wife of the Prophet Muhammad, used to convey the teachings of Islam to men and women alike. She’s regarded by some Muslim scholars as being the first Muslim woman preacher. The Quran is very clear in its laws regarding women. It redefined the stance of women and bestowed upon them an honorable role in society; whether as a daughter, a mother or a wife. Men are instructed in their fair and kind dealing towards women. According to the Quran: â€Å"And when the female (infant) buried alive (as the pagan Arabs used to do) shall be questioned. For what sin she was killed? † (Holy Quran, Surah At-Takwir 81:8-9). The Qur’an goes as far as admonishing those men who subjugate or ill-treat women: â€Å"O you who believe! You are forbidden to inherit women against their will. Nor should you treat them with harshness, that you may take away part of the dowry you have given them – except when they have become guilty of open lewdness. On the contrary live with them on a footing of kindness and equity. If you take a dislike to them, it may be that you dislike something and God will bring about through it a great deal of good†. (Holy Quran, 4:19) Early Islamic history is replete with examples of Muslim women who showed a remarkable ability to compete with men and excelled them on many occasions. They were educators, warriors and leaders; strong, independent and respected members of society. Unfortunately, that isn’t the case in most of the Muslim world today. Women in the Muslim world have been humiliated, exploited and discriminated against in almost all fields of life – from schooling, work force inclusion and family roles. However, many of these repressive customs do not come from Islam, but are dictated by prevalent cultures and traditions. Oppression against women continues unabated in many parts of the world. Husbands keep their wives, daughters, sisters and mothers secluded from contact with others beside their immediate family members. Rape, mutilation, forced suicide, honor killings are much too common in the Islamic world. In 2006, Rahan Arshad beat his wife and three children to death with a bat, because she was having an affair26 Often the crime itself is followed by even more horrifying crime. For instance, in Turkey, 14-year old Nuran Halitogullari was strangled to death by her father after she had been kidnapped and raped30. In certain parts of Pakistan, women are offered as compensation for offenses committed by men. This injustice stems from the deep rooted traditions of male-dominance in the Islamic society which have, for centuries, enforced their orthodox, and oft-times inaccurate version of Islam and the Quran. Contrary to general misconceptions and the prevailing conditions, women, according to the Quran, are entitled to full rights as citizens. Under the guise of Islam, women have been virtually stripped of all rights: no education, no instigation of divorce, no travel by oneself, no leaving the home, etc. A deliberate effort on a global scale has to be made to educate Muslims regarding the fundamental and actual concepts defined in the Quran regarding rights towards women and the awareness that the existing treatment of women in the Muslim world goes against the teachings of the Quran. Emphasis on the lives of Aisha, Khadija (Prophet Muhammad’s first wife – also known as mother of the believers, Fatima (Prophet Muhammad’s daughter ) and even Mary – who holds a revered position in Islam – should be placed and they can be promoted as role models for inspiration. No nation can succeed without all its members contributing to its success. Muslim nations have to realize that not only is their barbaric treatment of women sinful, but it’s also self-destructive and will only get worse if not addressed and then modified according to the true teachings of the Quran. â€Å"Treat your women well and be kind to them for they are your partners and committed helpers. â€Å"(Ismail 83). ? Works Cited Asthana, A. and Mistry. U. For Families that Fear Dishonor, There is Only One Remedy†¦ Murder. The Observer, 5 October, 2003. Bon, Gustave Le. The Arab Civilization. Paris: Firmin-Didot, 1884. Ismail, Imam Vehbi. Muhammad, the Last Prophet: The Last Prophet. Jordan: Amana

Mental Illness A World Full Of Confusion And...

To carry a mental illness, you carry a world full of confusion and misunderstandings. When someone has a mental illness, it impacts their thoughts, emotions, ability to relate to those around them, as well as functions in their daily lives. Because we can not see the problem, does not mean that there is not a problem. There are many people who has helped change and evolve ideas surrounding mental illnesses. There is a dark and sad history behind people who are mentally challenged, but the most progress of evolving the darkness surrounding it was a woman named Dorothea Dix (1802-1887). During her time, she volunteered to work in an all girls’ prison where she experienced watching the patients and prisoners be inhumanely treated (Parry, 1).†¦show more content†¦To whomever it is from, it creates a big impact in different ways. First off, stigma is consisted of four social cognitive processes called cues, stereotypes, prejudice, and discrimination (Cummings, Lucas, and D russ, 1). According to Cummings, Lucas, and Druss, cues are the labels, physical appearances, symptoms, social skill deficits and triggers the stereotypes; these are the negative structures that are related to mental illness, for instance, the idea that they are thought to be more violent in behavior. The stereotypes relates to the prejudice which is the rejection while discrimination is the behavior of the person rejecting someone with a mental illness (Cummings, Lucas, and Druss, 1). Adding on to the idea of stigma, there are two kinds, public stigma and self stigma. Public stigma is the societal response that the people have to the attributes of mental illnesses and can be a form of prejudice and behavioral reaction (Bathje, Pryor, 163-163). Self stigma is the psychological impact of possessing the characteristic and the impact of public stigma. Knowing what stigma is, one of the troubles that can occur because of it is the negative emotional response a person with psychological challenges goes through. Because of the prejudice and discrimination, it is a likely response for them to feel low self-esteem, shame, fear, and embarrassment, and because of the negative emotional response, it can lead to isolation or limiting their social